Expression of the Growth Hormone and Insulin-like Growth Factor 1 Genes in Male and Female Blue Gourami (trichogaster Trichopterus) at Different Temperatures

In this study, the GH and IGF-I of blue gourami (Trichogaster trichopterus) were examined at different temperatures of 23 ο C, 27 ο C and 31 ο C. GH mRNA expression in the pituitary of males and females at various temperatures was examined using RT-PCR. The mean of mRNA levels was higher in the pituitary glands of females maintained in 23 ο C and 27 ο C than in males. However, no significant difference in GH mRNA expression was observed in the pituitary glands of females maintained at 23 ο C, 27 ο C and 31 ο C (ANOVA p>0.05). The GH expression is high in females at 23 ο C and in males at 31 ο C. The mean of IGF-I expression in females was high at temperatures of 27 ο C and 31 ο C compared to 23 ο C in brain, liver and ovary, and only 27 ο C in muscle. In males, a high mean of IGF-I mRNA levels was detected in brain, liver and muscle at 27 ο C and at all three temperatures in the testis (23 ο C, 27 ο C and 31 ο C); the difference among mRNA levels was not significant. In males, a high mean of IGF-I mRNA levels was detected in brain, liver, ovary and muscle at 27 ο C but not in the testis. © 2014 Pak Publishing Group. All Rights Reserved.